CWbio™, GoldVac EndoFree Plasmid Maxi Kit, 10 preps
On demandCWbio™, GoldVac EndoFree Plasmid Maxi Kit, 10 preps
Endotoxin is a common contaminant in plasmid extraction. Because eukaryotic cells are very sensitive to endotoxin, the transfection efficiency will be greatly reduced if endotoxin is contained in the plasmid. This kit provides a simple, rapid and efficient method for the extraction of endotoxin-free plasmids.
100-300 ml of bacterial culture can be processed each time, and up to 2 mg of transfection-grade plasmid DNA can be obtained. Multiple samples can be handled simultaneously by use of a vacuum device and the whole process takes only 45 minutes, which effectively reduces hand-on time.
Based on conventional alkaline lysis method, the new and unique silicon membrane binds plasmid DNA efficiently and specifically. At the same time by using a special buffer system and endotoxin-removal filters, impurities such as endotoxin, genomic DNA, RNA, proteins are effectively removed.
The plasmids obtained from the kit have high purity and high yield and are particularly suitable for transfection experiments. The plasmids can also be used for DNA sequencing, PCR, in vitro transcription, restriction endonuclease digestion and other experiments
Contents
- Buffer P1: 125 ml
- Buffer P2: 125 ml
- Buffer E3: 125 ml
- Buffer PS: 30 ml
- Buffer PW (concentrated): 50 ml
- Endo-Free Buffer EB: 30 ml
- RNase A (10 mg/ml): 2 ml
- Plungers: 10
- Endo-Remover FQ: 10
- Spin Columns DQ with Collection Tubes: 10
- Centrifuge Tubes (50 ml): 10
Not included in the kit: 100% ethanol; Isopropanol; Vacuum and Pump apparatus.
MoreThere are no specifications
There are no report
You May Also Like
Invitrogen™ PureLink™ Quick Gel Extraction and PCR Purification Combo Kit
$ 188
In stock
Thermo Scientific™ GeneJET Whole Blood Genomic DNA Purification Mini Kit, 50 Preps
$ 243
In stock
Applied Biosystems™ ExoSAP-IT™ PCR Product Cleanup Reagent, 100 Reactions
$ 140
In stock
MGIEasy Swabs Viral Nucleic Acid Extraction Kit, MGISP-NE32 platform, 32Preps
$ On demand