Gibco™ DMEM/F-12
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
1 bottle x 500 mL
Order number :
11320033
Gibco™ DMEM/F-12
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications
Features
- With L-glutamine & Phenol Red
- Without HEPES
Using DMEM/F-12
- DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12
- This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.
- DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS).
- DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH
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