Edinburgh Genetics COVID-19 Colloidal Gold Immunoassay Testing Kit, IgG/IgM Combined, 50 pieces
On demandEdinburgh Genetics Colloidal Gold Immunoassay Testing Kit, IgG/IgM Combined is immunochromatography based
The test card contains (1) colloidal gold- labeled recombinant novel coronavirus antigen and quality control antibody colloidal gold marker, (2) two detection lines (G and M lines) and one quality control line (C) fixed on a nitrocellulose membrane. M is fixed with monoclonal anti-human IgM antibody for detecting the novel coronavirus IgM antibody. G is fixed with monoclonal antihuman IgG antibody for detecting the novel coronavirus IgG antibody. The quality control antibody is fixed on the C line. When an appropriate amount of test sample is added to the sample well of the test cassette, the sample will move forward along the test card via capillary action. If the sample contains IgM antibody, the antibody will bind to the colloidal gold labeled novel coronavirus antigen. The antibody/antigen complex will be captured by the anti-human IgM antibody immobilised on the membrane, forming a red M line and indicating a positive result for the IgM antibody. If the sample contains IgG antibodies, the antibody will bind to the colloidal gold-labeled novel coronavirus antigen and the antibody/antigen complex will be captured by the antibody immobilised on the membrane, forming a red G line and indicating a positive result for the IgG antibody. If neither antibody is present, a negative result is displayed. The card also contains a quality control line (C). Regardless of the antibodies present (or not) the C line should appear to indicate that the sample has been transported properly through the membrane. If the C line does not appear it indicates that the test result is invalid and a new, unopened test cassette is required to retest the sample.
Sample Requirements
- Suitable for human serum, plasma, or whole blood samples (capillary or venous) including samples prepared by commonlyused anticoagulants (EDTA, heparin, sodium citrate).
- Serum and plasma samples can be stored at 2-8°C for 5 days.
- If long-term storage of serum or plasma samples is required, store at -20°C and avoid repeated freeze/thaw cycles.
Contents
- One test kit contains: 20 Test Cassettes, 1 Package Insert.
- One test cassette contains: Dried reagents with stabilisers, Colloidal gold- labeled novel coronavirus antigen, Mouse anti-human IgG monoclonal antibody, Mouse anti-human IgM monoclonal antibody.
- Materials not included but required: Buffer Solution, Capillary Samplers, Lancet, Alcohol Wipes, Gloves, Timer.
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