EGFR 6-color Crystal Digital PCR™ Kit

EGFR 6-color Crystal Digital PCR™ Kit

The EGFR 6-color Crystal Digital PCR™ Kit allows for the detection and quantification of up to 32 somatic EGFR mutations in exons 18, 19, 20, and 21 associated with non-small cell cancer (NSCLC).

The EGFR 6-color Crystal Digital PCR™ Kit provides a fully validated, specific, and ultrasensitive assay for the 6-color naica® system. The kit can be used to test cell-free DNA (cfDNA) extracted from plasma samples.

The EGFR 6-color Crystal Digital PCR™ Kit is a ready-to-use solution comprised of six components, including the high-perfomance naica® multiplex PCR MIX 10X, an optimal primer and probe solution, a set of positive and negative controls, and water for sample dilution.

Features

• The EGFR 6-color Crystal Digital PCR™ kit reliably detects 32 common, rare, activating, and resistant somatic EGFR mutations in a single assay. 
• The EGFR 6-color Crystal Digital PCR™ kit is optimized for ultrasensitive and highly robust EGFR mutation detection on the naica® system with LoDs ranging from 0.30 to 0.46 cp/µL, depending on the target using Sapphire chips. 
• The Crystal Miner software and custom EGFR 6-color analysis template enable fast and straightforward analysis of cfDNA.

Contents

• Buffer A: naica® multiplex PCR MIX
• Buffer B: Reaction enhancer solution
• Buffer C: Ready to use primer and probe solution
• Positive control: Synthetic mutated DNA pool (concentrations defined in the Certificate of Compliance - CoC)
• Negative control
• Nuclease free water