Eppendorf BioSpectrometer® fluorescence

Eppendorf BioSpectrometer® fluorescence, 230 V/50 – 60 Hz

The Eppendorf BioSpectrometer fluorescence is an expansion of the Eppendorf BioSpectrometer basic and features an integrated fluorescence module. UV/Vis assays, fluorescence assays and semi-micro/micro-volume measurements can all be carried out on a single instrument without the need for external control (PC/Laptop). Eppendorf BioSpectrometer fluorescence can be used to increase the measuring range for biomolecules (nucleic acids and proteins) by a factor of 1,000, compared to UV measurements. Pre-programmed (modified) methods for Qubit DNA quantification kits (SS, HS, BR) expand the flexibility and functionality of the instrument. Additionally, the use of the Eppendorf µCuvette G1.0 for fluorescence assays (5 µL loading volume) allows for a vast reduction in reagent usage.

Ideal for

• NGS applications (micro-volume UV/Vis and fluorescence assays in a single instrument)
• dsDNA quantification down to 1 pg/µL with common kits, e.g., Pico Green®
• ssDNA quantification down to 10 pg/µL
• RNA quantification down to 20 pg/µL
• Oligo quantification down to 10 pg/µL
• Protein (BSA) quantification down to 0.01 ng/µL
• Quantification of GFP-binding proteins
• Growth measurement of GFP-producing bacteria

Features

• All Eppendorf BioSpectrometer basic options
• Preprogrammed and freely programmable fluorescence methods
• Preprogrammed (modified) methods for Qubit DNA quantification kits (SS, HS, BR)
• Fluorescence excitation wavelength 470 nm, emission wavelengths 520 nm and 560 nm
• Fluorescence intensity across a range of 0.5 nM to 2,000 nM fluorescein
• Single consumable (UVette) for UV/Vis and fluorescence assays