Gibco™ Defined Keratinocyte SFM (1X)

Gibco™ Defined Keratinocyte SFM (1X)

Defined Keratinocyte SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers. The growth-promoting activity of BPE has been replaced by the inclusion of growth-promoting agents, which enhance consistent product performance. These growth-promoting agents also maintain cell morphology, physiological markers, and growth. The medium is formulated to inhibit fibroblast proliferation and has a low calcium concentration (<0.1 mM), allowing for the isolation and culture of highly enriched populations of undifferentiated keratinocytes

Contents

• 500 mL Defined Keratinocyte-SFM Basal Medium; store in refrigerator (2–8°C) and protect from light
• 1 mL Defined Keratinocyte-SFM Growth Supplement; store in freezer (-5 to -20°C) and protect from light
• Complete Medium: store in refrigerator (2–8°C) and protect from light