Gibco™ Essential 8™ Adaptation Kit

Gibco™ Essential 8™ Adaptation Kit

The Essential 8™ Adaptation Kit includes Essential 8™ Medium and recombinant human Laminin 521 (rhLaminin-521) for optimal survival during transition from feeder-dependent to feeder-free culture. rhLaminin-521 has been proven to promote cellular survival under stressful conditions in the absence of small molecule inhibitors. In addition, rhLaminin-521 has been shown to support PSC growth for >20 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. rhLaminin-521 can also be purchased separately

Features

• Robust–transition cells between feeder-dependent and feeder-free culture systems with minimal effort
• Physiologically relevant–expand cells with minimal differentiation
• Scalable–passage stem cell lines as single cells with no impact to genetic stability

Contents

• 100 µg rhLaminin-521, store at -10 to -30°C, protect from light
• 500 mL basal medium, store at 2-8°C, protect from light
• 10 mL supplement, store at -5 to -20°C, protect from light