Gibco™ ExpiFectamine™ CHO Transfection Kit, For 1 L of culture
On demand
Order number :
A29129
Gibco™ ExpiFectamine™ CHO Transfection Kit, For 1 L of culture
The ExpiFectamine™ CHO Transfection Kit is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133). It is designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will power the highest possible level of protein expression from ExpiCHO-S™ Cells (Cat. No. A29127) cultured in ExpiCHO™ Expression Medium (Cat. No. A2910001).
Features
- Achieves greater than 90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
- Requires one-third to one-half less plasmid DNA than other transfection reagents while achieving superior expression performance
- Provides options for three transient expression protocols for maximal work flexibility while still fitting into the same overall workflow used by other transient expression systems, such as FreeStyle™ MAX CHO and Expi293™
- Enables robust and reproducible transfection results, giving you greater confidence in your results
- Demonstrates linear scalability of transfections from culture volumes of <1 mL to >10 L
- Transfection Technique: Lipid-Based Transfection
- Classification: Animal Origin-Free, Chemically Defined
- Cell Typr: Ovarian Cells, Established Cell Lines
Contents
- ExpiFectamine CHO Reagent: 3.2 mL
- ExpiFectamine CHO Enhancer: 6 mL
- ExpiCHO Feed: 330 mL