Gibco™ ExpiSf™ Expression System Starter Kit

Gibco™ ExpiSf™ Expression System Starter Kit

The ExpiSf Expression System is a complete chemically-defined baculovirus-insect cell protein expression system that delivers superior yields and consistent performance run after run using a fast, streamlined workflow.

Features

• A chemically defined, yeastolate-free, animal origin-free medium for consistent cell growth and protein expression
• Integrated components that work in concert to deliver three times higher protein yields compared to traditional insect expression platforms
• Robust production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
• A fast, simplified workflow that allows you to obtain protein in as little as 6–10 days; half the time of traditional protocols
• Scalable expression that can be applied to cultures from 24-deep well plates to >3 L shake flasks

Contents

• ExpiSf CD Medium: 1000 mL bottle 
• ExpiSf Enhancer: 4 x 1 mL 
• ExpiFectamine Sf Transfection Reagent: 1 mL 
• ExpiSf9 Cells: 2 x 1 vial 
• Opti-MEM: 500 mL
• pFastBac1 vector: 10 µg 
• pFastBac1-Gus control vector: 4 ng 
• MAX Efficiency DH10Bac Competent Cells: 5 x 0.1 mL 
• Single-Use Nalgene PETG Erlenmeyer Flasks: 24 x 125-mL