Gibco™ Human Melanocyte Basal Medium Kit, calcium free (formerly “Medium 254CF Kit”)

Gibco™ Human Melanocyte Basal Medium Kit, calcium free (formerly “Medium 254CF Kit”)

The Gibco Human Melanocyte Basal Medium Kit, Calcium Free (formerly “Medium 254CF Kit”), enables the culture of human epidermal melanocytes. is a basal medium containing essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. This medium does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. This medium is HEPES and bicarbonate buffered and is designed for use in an incubator with an atmosphere of 5% CO2/95% air. To support plating and long-term proliferation of normal human melanocytes, Human Melanocyte Basal Medium, Calcium Free, must be supplemented with calcium plus either Human Melanocyte Growth Supplement (HMGS, Cat. No. S-002-5) or PMA-Free Human Melanocyte Growth Supplement-2 (HMGS-2, Cat. No. S-016-5). Each of these supplements contains all of the growth factors, hormones, and tissue extracts necessary for growth of melanocytes in Human Melanocyte Basal Medium, Calcium Free.

Contents

• 500 mL Human Melanocyte Basal Medium, Calcium Free (formerly "Medium 254CF"); store in refrigerator (2–8°C) and protect from light
• Sterile stock solution of calcium chloride (0.2 M, 0.5 mL); store in refrigerator (2–8°C)