Gibco™ LV-MAX™ Lentiviral Production System

Gibco™ LV-MAX™ Lentiviral Production System

The Gibco LV-MAX Lentiviral Production System is the first optimized system that provides a scalable and high-yield lentiviral vector production platform. It is based on a suspension, high-density culture of HEK 293-derived Viral Production Cells adapted to a special chemically defined, serum-free and protein-free LV-MAX Production Medium. Optimal viral titer yield is mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel lentiviral-specific enhancer and supplement. All components work synergistically to generate superior and functional lentiviral particles under serum-free conditions compared to conventional polyethylenimine (PEI)

Features

• High titer—greater than 1 x 108 TU/mL (unconcentrated LVVs-GFP)
• Scalable suspension system—from 96 deep-well plate to 2-L bioreactor
• Serum-free—eliminating animal-origin components and associated risks
• Easy, robust culture and transfection protocols—fits into current transient production workflows

Contents

• 1 L LV-MAX Production Medium. Store at 2–8°C, protect from light.
• 0.3 L LV-MAX Transfection Kit. Store at 2–8°C, protect from light.
• 2 vials of Viral Production Cells. Store in liquid nitrogen until ready to use. Do not store the cells at -80°C.