Gibco™ rhLaminin-521, 100 µg

Gibco™ rhLaminin-521, 100 µg

Recombinant human Laminin 521 (rhLaminin-521) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8™ Medium, rhLaminin-521 has been proven to promote cellular survival under stressful conditions in the absence of small molecule inhibitors. rhLaminin-521 has been shown to support PSC growth for >20 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. rhLaminin-521 also provides optimum reprogramming efficiency of human somatic cells as well as human pluripotent stem cell survival during feeder-dependent to feeder-free transitions when used in conjunction with Essential 8 Medium. For a kit that combines the medium and substrate, see the Essential 8™ Adaptation Kit

Features

• Robust—transition cells between feeder-dependent and feeder-free culture systems with minimal effort
• Efficient—easily reprogram human fibroblasts to pluripotent stem cells
• Physiologically relevant—expand cells with minimal differentiation
• Scalable—passage stem cell lines as single cells with no impact to genetic stability