Gibco™ StemPro™ hESC SFM

Gibco™ StemPro™ hESC SFM

StemPro® hESC SFM is a fully defined, serum- and feeder-free medium (SFM) specially formulated for the growth and expansion of human embryonic (hESCs) and human induced pluripotent stem cells (iPSC). StemPro® hESC SFM has been extensively tested and is proven to maintain pluripotency in a growing list of hESC lines including BG01, BG02, BG03, H1, H9, and BG01V. In addition, StemPro® hESC SFM has been used to scale up production of hESCs to over 1 x 1010 cells. StemPro® hESC SFM has been shown to support hESC and iPSC growth for >50 passages without any signs of karyotypic abnormalities, and to maintain the ability of hESCs to differentiate into all three germ line lineages

Contents

• DMEM⁄F-12 with GlutaMAX™ medium
• Bovine serum albumin 25% (BSA)

Additional components required for complete medium (need to be purchased separately):

• FGF BASIC (full length) REC HU (Cat. no. PHG0261)
• 2-Mercaptoethanol