Gibco™ Advanced MEM, 10 x 500 mL
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
10 bottles x 500 mL
Order number :
12492021
Gibco™ Advanced MEM, 10 x 500 mL
Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced DMEM / F-12, with no adaptation, include PK-15, MDCK, Vero, WI-38 and Hep-2.
Features
- With Glucose, Non-Essential Amino Acids, Sodium pyruvate & Phenol Red.
- Without L-glutamine, HEPES.
- Gibco® Advanced MEM is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX® I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.
Using Advanced MEM
- Advanced MEM requires supplementation with 1-5% Fetal Bovine Serum and 4mM L-glutamine or GlutaMAX™ supplement.
- The FBS concentration must be optimized for each cell line to obtain maximum serum reduction.
- Many cell lines do not require adaptation to this media.
- Advanced MEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
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