Gibco™ BenchStable™ DMEM/F12, 500 mL

Gibco™ BenchStable™ DMEM/F12, 500 mL

Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications.

BenchStable DMEM/F-12 has been engineered for storage at room temperature while supporting cellular proliferation and maximum cell densities comparable to conventional DMEM/F12 when supplemented with ≥10% fetal bovine serum (FBS).

Features

• Optimized for room temperature stability.
• With GlutaMAX, Phenol Red.
• Without HEPEPS.
• This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.
• DMEM/F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Using BenchStable DMEM/F-12

• DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12.
• DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO₂ environment to maintain physiological pH.
• BenchStable DMEM/F-12 contains no proteins, lipids, or growth factors and so requires supplementation with ≥10% fetal bovine serum (FBS).