Gibco™ BenchStable™ RPMI 1640, 10 x 500 mL

Gibco™ BenchStable™ RPMI 1640, 10 x 500 mL

Gibco RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications.

BenchStable RPMI 1640 Medium has been engineered for storage at room temperature while supporting cellular proliferation and maximum cell densities comparable to conventional RPMI 1640 Medium when supplemented with ≥10% fetal bovine serum (FBS).

Features

• Optimized for room temperature stability.
• With GlutaMAX, Phenol Red.
• Without HEPES.
• RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins.
• RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium.
• In addition, the vitamins inositol and choline are present in very high concentrations.
• RPMI 1640 Medium with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Using BenchStable RPMI 1640 Medium

• BenchStable RPMI 1640 Medium contains no proteins, lipids, or growth factors and so requires supplementation with ≥10% fetal bovine serum (FBS).
• RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L) and so requires a 5–10% CO₂ environment to maintain physiological pH.