Gibco™ DMEM/F-12, Powder, HEPES, 1 L
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
1 bottle x 1 L
Order number :
42400010
Gibco™ DMEM/F-12, Powder, HEPES, 1 L
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco® DMEM / F-12 modifications for a range of cell culture applications.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation
Features
- With L-glutamine, HEPES & Phenol Red
- Without Sodium Bicarbonate
- Gibco® DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components.
Using DMEM/F-12
- DMEM /F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS).
- DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.
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