Gibco™ Ham's F-10 Nutrient Mix, HEPES, 500 mL

Gibco™ Ham's F-10 Nutrient Mix, HEPES, 500 mL

Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.

Features

• With Phenol Red, HEPES & L-glutamine.
• Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine.

Using Ham's F-10

• F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
• F-10 uses a sodium bicarbonate buffer system (1.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.