Gibco™ Ham's F-12 Nutrient Mix, Powder, 50 L
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
1 bottle x 50 L
Order number :
21700018
Gibco™ Ham's F-12 Nutrient Mix, Powder, 50 L
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco® F-12 modifications for a range of cell culture applications.
Features
- With L-glutamine & Phenol Red.
- Without Sodium Bicarbonate.
- Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine.
Using Ham’s F12
- Gibco® F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
- F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
- Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation.