Gibco™ Medium 199 (10X), 500 mL
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
1 bottle x 500 mL
Order number :
11825015
Gibco™ Medium 199 (10X), 500 mL
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications.
Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Features
- With Phenol Red.
- Without L-glutamine, HEPES & Sodium Bicarbonate.
- Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins.
Using Medium 199
- Medium 199 contains no proteins, lipids, or growth factors.
- Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
- Concentrated forms of Gibco® Medium 199 require sodium bicarbonate addition, pH adjustment, and filtration at the time of preparation.
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