Gibco™ Medium 199, Hanks' Balanced Salts, 500 mL

Gibco™ Medium 199, Hanks' Balanced Salts, 500 mL

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications.

This Medium 199 formulation contains Hanks' salts for use without CO₂.

Features

• With Hanks' salts, L-glutamine, HEPES & Phenol Red.
• Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins.

Using Medium 199

• Medium 199 contains no proteins, lipids, or growth factors. Therefore, Medium 199 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
• This Medium 199 formulation does not require a CO₂ environment to maintain physiological pH.