Gibco™ Medium NCTC-109 (1X), 500 mL

Gibco™ Medium NCTC-109 (1X), 500 mL

NCTC-109 medium is a chemically defined medium that was originally developed by the National Cancer Institute and formulated for the culture of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment. Gibco® NCTC-109 medium is also suitable for generating and maintaining hybridoma cells.

Features

• With Phenol Red & Coenzymes
• Without L-glutamine & HEPES
• Gibco® NCTC-109 medium is rich in nucleosides, vitamins, coenzymes, reducing agents and other unique ingredients

Using NCTC-109 medium

• NCTC-109 contains no proteins, lipids, or growth factors. NCTC-109 medium typically requires supplementation of a growth factor cocktail (for serum free growth) or Fetal Bovine Serum (FBS)
• NCTC-109 medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH