Gibco™ MEM α, GlutaMAX™ Supplement, Nucleosides, 10 x 500 mL

Gibco™ MEM α, GlutaMAX™ Supplement, Nucleosides, 10 x 500 mL

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco® MEM α modifications for a range of cell culture applications.

This product is made with Earle’s salts.

Features

• With Phenol Red, GlutaMAX™, Ribonucleosides & Deoxyribonucleosides.
• MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid.
• GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Using MEM α

• MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
• MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.