Gibco™ MEM (Temin's modification) (2X), No Phenol Red, 250 mL

Gibco™ MEM (Temin's modification) (2X), No Phenol Red, 250 mL

Temin's Modified Eagle Medium, (MEM) is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco® MEM modifications for a range of cell culture applications.

This MEM formulation contains Earle's salts for use in a CO₂ incubator.

Features

• With L-glutamine & Sodium Bicarbonate.
• Without HEPES & Phenol Red.
• Temin's Modification is a 2x MEM with 4x the vitamins and amino acids of the original MEM.

Using MEM (Temin's modification)

• MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
• MEM uses a sodium bicarbonate buffer system (8.4 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.