Gibco™ RPMI 1640 Medium, 500 mL

Gibco™ RPMI 1640 Medium, 500 mL

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications.

Features

• With L-glutamine & Phenol Red.
• Without HEPES.
• Gibco® RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins.
• RPMI 1640 contains biotin, vitamin B₁₂, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium.
• In addition, the vitamins inositol and choline are present in very high concentrations.

Using RPMI 1640

• RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
• RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.