Gibco™ RPMI 1640 Medium, Low HEPES, Low Bicarbonate, No Glutamine, 500 mL
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
1 bottle x 500 mL
Order number :
42402016
Gibco™ RPMI 1640 Medium, Low HEPES, Low Bicarbonate, No Glutamine, 500 mL
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications.
Features
- With HEPES & Phenol Red.
- Without L-glutamine.
- Gibco® RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins.
- RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium.
- In addition, the vitamins inositol and choline are present in very high concentrations.
Using RPMI 1640
- RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
- RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
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