Gynemed SemenVIT, Reagent 2

Gynemed SemenVIT, Reagent 2

For Assessment of Ejaculate. Gynemed SemenVIT, Reagent 2 is a component of Gynemed SemenVIT

This SemenVit kit is performed to examine the vitality of sperm cells. It is particularly important in semen samples with less than 40% of forward moving (motile) sperms.

The determination of the vitality of sperm cells is judged by the integrity of the sperm membrane. The dye exclusion method is based on the fact that dead sperms with damaged plasma membranes absorb certain dyes.

Evaluation: Vital sperms appear colorless, transparent or light pink; dead or not viable cells are stained red. Sometimes in sperms within the colored neck

region and not colored head and flagellum are observable- This caused by damage to the membrane and these sperm are classified as vital.

Count 200 cells and distinguish between vitalsperms from dead sperms. The total number ofmembrane-intact sperms is of biological significance. The value is determined by multiplying the total number of sperms in the ejaculate by the percentage of membrane-intact cells.

Total number of vital sperms = Total number of sperms x percentage vital sperms