Gynemed® ZyMōt chamber, ICSI

Gynemed® ZyMōt chamber, ICSI

The ZyMōt chambers from DxNow maybe known to you under the name FERTILE and FERTILE Plus. With the new name ZyMōt they available from us now (or we can help you to get into contact with your distributor).

The ZyMōt chambers are an alternative to the usual sperm preparation which includes the centrifugation. They are easy to use, easy to establish in the lab and save a lot of time. But most importantly the procedure is very gentle for the sperm cells and enables enrichment of progressively motile sperm with significantly reduced DNA fragmentation1.

The ZyMōt is available in three versions. The ZyMōt Multi (850µl) is the most commonly used chamber. This chamber can be used for sperm preparation for ICSI, IUI and IVF. Handling is very easy, since the native ejaculate is injected into the chamber without prior preparation. In the chamber there is a special membrane with micropores through which the motile sperm migrate. This membrane prevents damaged sperm from passing through. A suitable handling medium is applied above the membrane (e.g. GM501 Sperm­Air) in which the sperm cells that have overcome the membrane accumulate. After 30 minutes of incubation, this supernatant is removed and the retrieved spermatozoa can be used directly for the desired application (ICSI, IUI or IVF). According to the manufacturer, the time spent actively preparing sperm is reduced to less than five minutes.

ZyMōt ICSI and ZyMōt Multi Sperm Separation Devices are used to prepare motile sperm for assisted reproductive technology (ART) procedures. Both devices separate sperm based on motility. The ZyMōt ICSI and the ZyMōt Multi are sterile and single use only. The mechanism of action for both is separation of sperm based on motility within a microenvironment created by the micro-channels of the ZyMōt ICSI or the micro-pores in the filter of the ZyMōt Multi. The primary difference between the devices is the processing volume. The ZyMōt ICSI has a processing volume of 2µl per micro-channel. The ZyMōt Multi is manufactured in two (2) processing volumes, 850µl and 3ml.

1- The ZyMōt ICSI has 5 micro-channels; each accommodating 2µl of semen. More than one micro-channel is available to accommodate multiple separations. Each channel has an inlet port for applying the semen sample and an outlet port for collecting the motile sperm. The ports are connected by a fluid-filled micro-channel in which the separating occurs. Untreated semen is added through the inlet port. After a period of time, the separated sperm are collected from the outlet port.

2- The ZyMōt Multi (provided with 850µl and 3ml collection chambers) has an inlet port that communicates with the lower sample chamber. The sample chamber is separated from the upper collection chamber by a microporous filter. Untreated semen is added through the inlet port. After a period of time, the separated sperm are collected from the upper chamber through the outlet port.

Indications for Use:

The ZyMōt ICSI Sperm Separation Device is intended for preparing motile sperm from semen for use in the treatment of infertile couples by intracytoplasmic sperm injection (ICSI) procedures.

The ZyMōt Multi (850µl) Sperm Separation Device is intended for preparing motile sperm from semen for use in the treatment of infertile couples by intracytoplasmic sperm injection (ICSI), in vitro fertilization (IVF) and intrauterine insemination (IUI) procedures.