Invitrogen 100 µg CD44 Monoclonal Antibody (IM7), PE-eFluor 610, eBioscience™

Invitrogen 100 µg CD44 Monoclonal Antibody (IM7), PE-eFluor 610, eBioscience™

The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.

• PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
• Fixation: Samples can be stored in IC Fixation Buffer (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Filtration: 0.2 µm post-manufacturing filtered.
• Recommended Isotype Control: Rat IgG2b kappa Isotype Control (eB149/10H5), PE-eFluor 610, eBioscience™.