Invitrogen 100 µg MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Alexa Fluor 532, eBioscience™
On demand
Order number :
58-5321-82
Invitrogen 100 µg MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Alexa Fluor 532, eBioscience™
The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
- Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
- Recommended Isotype Control: Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor 532, eBioscience™
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