Invitrogen 100 µg MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Super Bright 436, eBioscience™

Invitrogen 100 µg MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Super Bright 436, eBioscience™

The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.

• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
• Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser, Please make sure that your instrument is capable of detecting this fluorochrome.
• Recommended Isotype Control: Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright 436, eBioscience™