Invitrogen 25 µg Ki-67 Monoclonal Antibody (SolA15), APC-eFluor 780, eBioscience™
On demandInvitrogen 25 µg Ki-67 Monoclonal Antibody (SolA15), APC-eFluor 780, eBioscience™
The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin).
- APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
- Fixation: Samples can be stored in IC Fixation Buffer (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
- Filtration: 0.2 µm post-manufacturing filtered.
- Recommended Isotype Control: Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor 780, eBioscience™
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