Invitrogen 25 mg MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Functional Grade, eBioscience™

Invitrogen 25 mg MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Functional Grade, eBioscience™

The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.

• Applications: Neutralization (Neu), Functional Assay (FN), Immunomicroscopy (IM), Inhibition Assays (IA).
• Filtration: 0.2 µm post-manufacturing filtered.
• Purity: Greater than 90%, as determined by SDS-PAGE.
• Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
• Aggregation: Less than 10%, as determined by HPLC.
• Recommended Isotype Control: Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™