Invitrogen 4-1BB (CD137/TNFRSF9) Mouse ELISA Kit, 5 x 96 Tests
On demandInvitrogen 4-1BB (CD137/TNFRSF9) Mouse ELISA Kit, 5 x 96 Tests
The Mouse 4-1BB (CD137/TNFRSF9) ELISA quantitates Ms 4-1BB in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms 4-1BB.
Principle of the method
The Mouse 4-1BB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Interassay CV: <12%
- Intraassay CV: <10%
- Homogenous (no wash): No
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Mouse 4-1BB Antibody Coated wells, 96-well plate, 1 plate
- Mouse 4-1BB Biotin Conjugate, 2 vials
- Mouse 4-1BB Standard, recombinant mouse 4-1BB, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent C, 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (200X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
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