Invitrogen 50 µg Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), PE-Cyanine7, eBioscience™

Invitrogen 50 µg Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), PE-Cyanine7, eBioscience™

The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.

• Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
• Fixation: Samples can be stored in IC Fixation Buffer (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
• Filtration: 0.2 µm post-manufacturing filtered.
• Applications: Functional Assay (FN).
• Recommended Isotype Control: Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™