Invitrogen 6Ckine/CCL21 Human ELISA Kit, 5 x 96 Tests
On demandInvitrogen 6Ckine/CCL21 Human ELISA Kit, 5 x 96 Tests
The Human 6Ckine/CCL21 ELISA quantitates Hu 6Ckine in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu 6Ckine.
Principle of the method
The Human 6Ckine solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: <12%
- Intraassay CV: <10%
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Human CCL21 Antibody Coated wells, 96-well plate, 1 plate
- Human CCL21 Biotin Conjugate, 2 vials
- Human CCL21 Standard, recombinant human CCL21, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent C, 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (400X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
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