Invitrogen™ AbC™ Anti-Mouse Bead Kit

Invitrogen™ AbC™ Anti-Mouse Bead Kit

The AbC™ Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies with any laser in flow cytometry.

Features

• Species reactivity, The AbC™ Anti-Mouse Bead Kit is ideal for compensation with all isotypes of mouse (IgG1, IgG2a, IgG2b, IgG3, IgM) immunoglobulins.
• Maximum laser compatibility, All AbC™ compensation bead kits are ideal for use with any excitation source, including the 405 nm laser which has historically provided higher background signals due to auto-fluorescence with other kits on the market.

How it works

Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads). After mixing with a fluorophore-conjugated mouse antibody, the two components provide a distinct high-signal positive control with an appropriate negative population that can then be used to set compensation properly regardless of the intensity of the cells in the actual experiment.

Detection Method, Fluorescent.

Excitation Wavelength Range, any lasers (UV to 633/635). 

Stain Configuration, Surface.

Content

• 1 vial AbC™ capture beads
• 1 vial of negative beads