Invitrogen™ Adiponectin Human ELISA Kit, HRP, 96 Tests

 Invitrogen™ Adiponectin Human ELISA Kit, HRP, 96 Tests

The Human Adiponenctin ELISA quantitates Hu Adiponectin in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Adiponectin.

Principle of the method

The Human Adiponnectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features

• Homogenous (no wash): No
• Interassay CV: 5.8%
• Intraassay CV: 3.5%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Hu Adiponectin Standard, lyophilized (64.0 ng recombinant Hu adiponectin), 1 vial
• ELISA Buffer (10X), 2 x 30 mL
• Antibody Coated Wells, 96-well plate, 6 × 16-well strip
• Hu Adiponectin Detection Antibody (1000X), 30 μL
• Anti-Rabbit IgG HRP (100X), 150 μL
• Wash Buffer Concentrate (10X), 2 x 30 mL
• TMB Substrate Solution, 12 mL
• Stop Solution, 12 mL
• Adhesive Plate Covers, 2 each