Invitrogen AKT1 (Phospho) [pS473] Human ELISA Kit, Ultrasensitive, 96 Tests
On demandInvitrogen AKT1 (Phospho) [pS473] Human ELISA Kit, Ultrasensitive, 96 Tests
The Human AKT1 (Phospho) [pS473] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of AKT1 (Phospho) [pS473] in fresh or frozen human cell lysates. Cross-reactivity with mouse and rat cells has been observed. The assay recognizes both natural and recombinant AKT1 (Phospho) [pS473].
Principle of the method
A
monoclonal capture antibody specific for AKT1 (Phospho) [pS473] has
been coated onto the wells of the 96-well plate. During the first
incubation, standards of known content and unknown samples are pipetted
into the wells and the antigen binds to the immobilized (capture)
antibody. After washing, a rabbit antibody specific for the target
protein is added to the wells and serves as a detection antibody by
binding to the immobilized protein captured during the first incubation.
After washing, a horseradish peroxidase labeled anti-rabbit IgG is
added. This binds to the detection antibody to complete the four member
sandwich. After a third incubation and washing to remove all the unbound
enzyme, a substrate solution (TMB) is added, which is acted upon by the
bound enzyme to produce color. The intensity of this colored product is
directly proportional to the concentration of target protein present in
the original specimen and the optical density can be read on a standard
microplate reader.
Features
- Homogenous (no wash): No
- Interassay CV: 5.2%
- Intraassay CV: 4.7%
- Shipping condition: Wet Ice
- Storage: 2-8°C
Contents
- AKT1 [pS473] Standard, lyophilized; contains 0.1% sodium azide. 2 vials
- Standard Diluent Buffer; contains 0.1% sodium azide, red dye[1], 25 mL
- Antibody Coated Wells; 96-well plate, 1 plate
- AKT1 [pS473] Detection Antibody; contains 0.1% sodium azide, blue dye[1], 6 mL
- Anti‑Rabbit IgG HRP (100X), 0.125 mL
- HRP Diluent; contains 3.3 mM thymol, yellow dye[1], 25 mL
- Wash Buffer Concentrate (25X), 100 mL
- Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
- Stop Solution, 25 mL
- Adhesive Plate Covers, 3
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