Invitrogen™ Alexa Fluor™ 546 Antibody Labeling Kit
On demand
Order number :
A20183
Invitrogen™ Alexa Fluor™ 546 Antibody Labeling Kit
Molecular Probes® Alexa Fluor® Antibody Labeling Kits provide a convenient means to label small amounts of antibodies with Alexa Fluor® dyes (choice of 10 colors). This kit is optimized for labeling 100 µg of antibody per reaction with orange fluorescent Alexa Fluor® 546. Comparably small amounts of other proteins (>40 kDa) can also be labeled.
The kit contains everything you need to perform five separate labeling reactions as well as to purify the resulting conjugates. Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, indirect FISH, and more.
Features
- With an excitation and emission maximum of 554/570 nm, Alexa Fluor®546 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3® filters
- Labeled proteins typically ready to use typically in 90 min (∼15 min hands-on time)
- Useful for labeling 100 µg of protein
- Optimized for small-scale labeling of any protein >40 kDa
- Purified using convenient spin filters
- Stabilizing proteins must be removed from the sample before labeling
- Includes detailed instructions for determining degree of labeling (DOL)
Contents
- Alexa Fluor™ 546 Antibody Labeling Kit comes with 5 vials of reactive dye, sodium bicarbonate, purification resin, spin columns and collections tubes.
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