Invitrogen Allopregnanolone ELISA Kit, 96 Tests
On demandInvitrogen Allopregnanolone ELISA Kit, 96 Tests
The Allopregnanolone Competitive ELISA quantitates allopregnanolone in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.|Principle of the method: The Progesterone Metabolites Competitive ELISA research-use-only kit is designed to quantitatively measure allopregnanolone independent of species. A allopregnanolone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit allopregnanolone antibodies and allopregnanolone-peroxidase conjugate is added to the wells. The allopregnanolone-peroxidase conjugate and any allopregnanolone in the sample will compete to bind to the anti-rabbit allopregnanolone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound allopregnanolone-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of allopregnanolone present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Features
- Interassay CV: 8 %
- Intraassay CV: 7 %
- Homogenous (no wash): No
- Shipping condition: Wet or Dry Ice
- Storage: -20°C
Contents
- Anti-IgG coated 96 well plate
- Standard
- Assay Buffer Concentrate
- Capture Antibody
- HRP-conjugated Antigen
- Wash Buffer Concentrate
- TMB Substrate
- Stop Solution
- Plate Sealer
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