Invitrogen Alpha Synuclein Human ELISA Kit, 96 Tests
On demandInvitrogen Alpha Synuclein Human ELISA Kit, 96 Tests
The Human alpha Synuclein ELISA quantitates Hu alpha Synuclein in human cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Hu alpha Synuclein.
Principle of the method
The Human alpha Synuclein solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Interassay CV: 6.4%
- Intraassay CV: 5.7%
- Homogenous (no wash): No
- Shipping condition: Wet Ice
- Storage: 2-8°C
Contents
- Hu α-Synuclein Standard; contains 0.1% sodium azide, 2 vials
- Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
- Antibody Coated Plate; 96-well plate, 1 plate
- Hu α-Synuclein Detection Antibody; contains 0.1% sodium azide, blue dye [1], 6 mL
- Anti-Rabbit IgG HRP (100X); contains 3.3 mM thymol, 0.125 mL
- HRP Diluent; contains 3.3 mM thymol, yellow dye [1], 25 mL
- Wash Buffer Concentrate (25X), 100 mL
- Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
- Stop Solution, 25 mL
- Adhesive Plate Covers, 3
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