Invitrogen™ Ambion™ T4 RNA Ligase, cloned, 5 U/µL

Invitrogen™ Ambion™ T4 RNA Ligase, cloned, 5 U/µL

Ambion™ T4 RNA Ligase catalyzes the formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor. Substrates include RNA, DNA, and oligonucleotides. One tube containing 2,500 U (5 U/ µL) is provided along with 10X Reaction Buffer. The enzyme can be used for tagging the 5' ends of mRNA with oligonucleotides for mapping studies (RACE), 3'-end labeling RNA molecules, and circularizing RNA and DNA molecules.

Contents

• T4 RNA Ligase and 10X Reaction Buffer should be stored at –20°C.