Invitrogen Amyloid beta 42 Mouse ELISA Kit, 96 Tests

Invitrogen Amyloid beta 42 Mouse ELISA Kit, 96 Tests

The Mouse Amyloid Beta 42 (Ms Abeta 42) ELISA quantitates Ms Abeta 42 in cerebrospinal fluid, tissue and cell culture supernatant. The assay will exclusively recognize both natural and recombinant Ms Abeta 42. Cross-reactivity has been observed in rat samples.

Principle of the method

The Mouse Abeta 42 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Interassay CV: 7.1%
• Intraassay CV: 5.9%
• Homogenous (no wash): No
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Ms Aβ42 Standard, lyophilized synthetic peptide; contains 0.1% sodium azide, 1 vial
• Standard Diluent Buffer; contains 0.1% sodium azide, red dye [1], 25 mL
• Antibody Coated Wells. 96-well plate, 1 plate
• Ms Aβ42 Detection Antibody; contains 0.1% sodium azide, blue dye [1], 11 mL
• Anti-Rabbit IgG HRP (100X), 0.125 mL
• HRP Diluent; contains 3.3 mM thymol, yellow dye [1], 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Adhesive Plate Covers, 2