Invitrogen Amyloid Precursor Protein Human ELISA Kit, 96 Tests

Invitrogen Amyloid Precursor Protein Human ELISA Kit, 96 Tests

The Human Amyloid Precursor Protein (APP) ELISA quantitates Hu APP in human cerebrospinal fluid and cell lysates. The assay will exclusively recognize both natural and recombinant Hu APP.

Principle of the method

The Human APP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Interassay CV: 7.4%
• Intraassay CV: 6.3%
• Homogenous (no wash): No
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• Hu APP Standard, lyophilized; contains 0.1% sodium azide, 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
• Antibody Coated Wells, 96-well plate, 1 plate
• Hu APP Biotin Conjugate; contains 0.1% sodium azide, 11 mL
• Streptavidin HRP (100X), 0.15 mL
• Streptavidin HRP Diluent; contains 3.3 mM thymol, 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Plate Covers, adhesive strips, 3