Invitrogen™ Anza™ DNA End Repair Kit

Invitrogen™ Anza™ DNA End Repair Kit

The Invitrogen™ Anza™ DNA End Repair Kit is used to convert DNA with cohesive ends to blunt-ended 5’-phosphorylated DNA for use in blunt-end ligation reactions. The included pre-mixed Anza™ DNA End Repair Mix contains T4 DNA polymerase, Klenow Fragment, and T4 PNK (polynucleotide kinase) to minimize pipetting, while the Anza™ 10X End Repair Buffer contains ATP and dNTPs to facilitate the activity of the enzyme mix.

Features

• Convenient—pre-mixed enzyme mixture for superior convenience and reduction of pipetting
• Efficient—simultaneous blunting and phosphorylation
• Compatible—use directly in Anza restriction enzyme digestion reaction mixture following heat inactivation
• Fast—end repair complete in 15 minutes

Contents

• 100 µL Anza DNA End Repair Mix
• 200 µL Anza 10X End Repair Buffer