Invitrogen Atrial Natriuretic Peptide (ANP) Competitive ELISA Kit, 96 Tests

Invitrogen Atrial Natriuretic Peptide (ANP) Competitive ELISA Kit, 96 Tests

The Atrial Natriuretic Peptide (ANP) Competitive ELISA quantitates ANP in human plasma, urine or cell culture medium.

Principle of the method

The Atrial Natriuretic Peptide (ANP) Competitive ELISA research-use-only kit is designed to quantitatively measure ANP present in plasma, urine, and tissue culture media samples. This kit is not recommended for serum or plasma samples without extraction. An ANP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An ANP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of an antibody to ANP to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound ANP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm. 

Features 

• Interassay CV: 7.6%
• Intraassay CV: 6.7%
• Homogenous (no wash): No
• Shipping condition: Wet or Dry Ice
• Storage: -20°C

Contents

• ANP Standard; 1,800 ng/mL ANP in a special stabilizing solution, 125 μL
• Assay Buffer Concentrate (5X), 28 mL
• Antibody Coated Wells, 96-well strip-well plate coated with goat anti-rabbit IgG, 1 plate
• ANP Antibody, 3 mL
• ANP Conjugate, 3 mL
• Extraction Solution, 50 mL
• Wash Buffer Concentrate (20X), 30 mL
• TMB (Tetramethylbenzidine) Substrate, 11 mL
• Stop Solution; c ontains 1 M HCl, CAUSTIC, 5 mL
• Plate Sealer, 1