Invitrogen BAFF Human Instant ELISA™ Kit, 96 Tests

Invitrogen BAFF Human Instant ELISA™ Kit, 96 Tests

The Human B cell Activating Factor belonging to the TNF Family (Hu BAFF) ELISA quantitates Hu BAFF in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu BAFF.

Principle of the method

The Human BAFF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Interassay CV: 9.8%
• Intraassay CV: 8.2%
• Homogenous (no wash): No
• Storage: -20°C

Contents

• Pre-coated 96 well Microplate
• Biotinylated Detection Antibody
• SAV-HRP
• Sample Diluent, lyophilized
• Standard (8-well strips x 4)
• Wash Buffer Concentrate
• Substrate Solution
• Sample Diluent
• Stop Solution
• Lysis Buffer 10x
• Adhesive Plate Covers