Invitrogen BCL-W (BCL2L2) Rat ELISA Kit, 10 x 96 Tests
On demandInvitrogen BCL-W (BCL2L2) Rat ELISA Kit, 10 x 96 Tests
The Rat BCL-W (BCL2L2) ELISA quantitates Rt BCL-W in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt BCL-W.
Principle of the method
The Rat BCL-W solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Features
- Interassay CV: <12%
- Intraassay CV: <10%
- Homogenous (no wash): No
- Shipping condition: Wet or Dry Ice
- Storage: 2-8°C
Contents
- Rat BCL-W Antibody Coated wells, 96-well plate, 1 plate
- Rat BCL-W Biotin Conjugate, 2 vials
- Rat BCL-W Standard, recombinant rat bcl-w, 2 vials
- Wash Buffer Concentrate (20X), 25 mL
- Assay Diluent A (contains 0.09% sodium azide), 30 mL
- Assay Diluent B (5X), 15 mL
- Streptavidin-HRP (500X), 0.2 mL
- TMB Substrate, 12 mL
- Stop Solution, 8 mL
- Adhesive Plate Covers, 2
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