Invitrogen™ Bluo-Gal, substrate for β-galactosidase

Invitrogen™ Bluo-Gal, substrate for β-galactosidase

Bluo-gal (halogenated indolyl-β-galactoside) is an alternative histochemical substrate for β-galactosidase that produces a darker blue color than X-gal, the more frequently used substrate. β-galactosidase hydrolyzes bluo-gal to produce a blue precipitate that is insoluble in alcohol and xylenes and is suitable for immunoblotting and immunocytochemical assays. Bacteria containing active β-galactosidase produce blue colonies when grown on plates containing bluo-gal. Bluo-gal is soluble in dimethylformamide.